Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using the media selector tool.

This Advanced DMEM/F-12 is manufactured as follows:

With Without

• Non-essential amino acids; • Glucose;

• Sodium pyruvate; • Phenol red;

  • L-arginine;

  • L-glutamine;

  • L-lysine;

  • HEPES;

Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System:

SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.